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ADSL polyclonal antibody

货号: BS8096

价格：￥ ——

货期：现货（1-2个工作日）

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No. :BS8096

Product Name :ADSL polyclonal antibody

Swiss-Prot :P30566

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB ELISA IHC IF

Application_all :WB: 1:500-1:2000 ELISA: 1:5000-1:10000 IHC: 1:100-1:500 IF: 1:100-1:500

Background :ADSL (adenylosuccinate lyase), also known as AMPS, ASL or ASASE, is a 484 amino acid protein that is involved in both purine biosynthesis and in the formation of adenosine monophosphate (AMP) from inosine monophosphate. Expressed ubiquitously, ADSL catalyzes two key reactions in AMP biosynthesis, namely the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and the subsequent removal of fumarate from adenylosuccinate to yield AMP. Defects in the gene encoding ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency), an autosomal recessive disorder characterized by epilepsy, growth retardation and muscular wasting. Multiple isoforms of ADSL exist due to alternative splicing events.

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ADSL polyclonal antibody detects endogenous levels of ADSL protein.

BiowMW :~ 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Adenylosuccinate lyase; ASL; Adenylosuccinase; Asase; ADSL; AMPS

Immunogen :KLH conjugated synthetic peptide derived from human Adenylosuccinate Lyase: 185-280/484

Conjugate :Unconjugated

Modification :Unmodification

Primary: Anti-Adenylosuccinate Lyase at 1/1000 dilution

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenylosuccinate Lyase) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenylosuccinate Lyase) Polyclonal Antibody, Unconjugated .at 1:200 overnight at 4°C.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenylosuccinate Lyase) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.