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Pan-Crotonyl-K polyclonal antibody

货号: BS74126

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS74126

Product Name :Pan-Crotonyl-K polyclonal antibody

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Lysine crotonylation (Kcr) was first discovered about 10 years ago, as a PTM of histone. It was revealed that Kcr is an evolutionarily conserved and common PTM that occurs in both core histone and some non-histone proteins in a variety of organisms. Similar to other types of PTM, Kcr is a reversible modification. The classic histone acetyltransferases (HATs), p300/CBP, PCAF, and MOF, are responsible for most crotonylation events, while the histone deacetylases (HDACs) HDAC1/2/3 and SIRT1/2/3 reverse these reactions . The chromodomain protein CDYL acts as a crotonyl-CoA hydratase to negatively regulate histone Kcr by reducing substrate supply. CDYL-regulated Kcr of RPA1 plays an important role in homologous recombination (HR)-mediated DNA repair (23), while HDAC-regulated histone crotonylation is reduced after DNA damage .

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Other Modified

BiowMW :10-250KDa

Note :For research use only, not for use in diagnostic procedure.

Immunogen :A synthetic peptide corresponding to a sequence containing acetylatedK.

Conjugate :Unconjugated

Modification :Other Modified

Western blot analysis of extracts of various cell lines, using Pan-Crotonyl-K antibody at 1:1000 dilution.HeLa and NIH/3T3 cells were treated by Sodium crotonate for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Western blot analysis of extracts of various cell lines, using Pan-Crotonyl-K antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.