产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

EGFR polyclonal antibody

货号: BS72285

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS72285

Product Name :EGFR polyclonal antibody

Swiss-Prot :P00533

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB,IHC,IF

Application_all :WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background :The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :EGFR polyclonal antibody detects endogenous levels of EGFR protein.

BiowMW :~ 180kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling,

Alternative Name :Epidermal growth factor receptor; EC=2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; EGFR

Immunogen :A synthetic peptide of human EGFR

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using EGFR antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human lung cancer tissue using EGFR antibody at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug EGFR antibody.

Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer

JournalInternational Journal Of Cancer

ApplicationIP,WB,IHC

IF5.145

SpeciesHuman

PMID25546612