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ALKBH8 polyclonal antibody

货号: BS70772

价格：￥ ——

货期：现货（1-2个工作日）

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No. :BS70772

Product Name :ALKBH8 polyclonal antibody

Swiss-Prot :Q96BT7

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB IHC IF

Application_all :WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100

Background :ALKBH8 (alkylated DNA repair protein AlkB homolog 8) is a 664 amino acid protein that is encoded by a gene located on chromosome 11. ALKBH8 contains one RRM (RNA recognition motif) domain and belongs to the AlkB family of proteins. ALKBH8 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecular oxygen as the oxidizing agent. Three isoforms exist for ALKBH8 due alternative splicing of the gene.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ALKBH8 polyclonal antibody detects endogenous levels of ALKBH8 protein.

BiowMW :~ 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ABH8;

Immunogen :Recombinant protein of human ALKBH8.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry of paraffin-embedded mouse lung using ALKBH8 antibody at dilution of 1:100 (x40 lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using ALKBH8 antibody at dilution of 1:100 (x40 lens).

Immunofluorescence analysis of A549 cell using ALKBH8 antibody. Blue: DAPI for nuclear staining.

免疫沉淀（IP）