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Cleaved-DFF45 (D224) polyclonal antibody（KU）

货号: BS7010

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7010

Product Name :Cleaved-DFF45 (D224) polyclonal antibody（KU）

Swiss-Prot :O00273

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADphospho-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca2+-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cleaved-DFF45 (D224) polyclonal antibody detects endogenous levels of Cleaved-DFF45 (D224) protein.

BiowMW :~ 35, 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; Endonuclease VIII-like 3; Nei-like protein 3; NEIL3

Immunogen :Synthetic peptide, corresponding Human NEIL3.

Conjugate :Unconjugated

Modification :Cleaved

Western blot (WB) analysis of Cleaved-DFF45 (D224) polyclonal antibody at 1:500 dilutionLane1:HCT116 whole cell lysate(40ug)Lane2:LOVO whole cell lysate(40ug)Lane3:CT26 whole cell lysate(40ug)Lane4:PC12 whole cell lysate(40ug)Lane5:EC9706 whole cell lysate(40ug)