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Histone H3 (Acetyl-K18) polyclonal antibody

货号: BS70006

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS70006

Product Name :Histone H3 (Acetyl-K18) polyclonal antibody

Swiss-Prot :P68431/Q71DI3/P84243

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:200

Background :Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Ac-Histone H3 (K18) polyclonal antibody detects endogenous levels of Histone H3 protein only when acetylated at Lys18.

BiowMW :~ 17 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Histone Lysine Methylation Pathway,Examples of Crosstalk Between Post-translational Modifications,DNA Methylation,

Alternative Name :Histone H3.1; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.2; Histone H3/m; Histone H3/o; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.3; H3F3A; H3.3A; H3F3; H3F3B; H3.3B

Immunogen :Synthetic peptide including acetyl-lysine 18 of Histone H3.

Conjugate :Unconjugated

Modification :Acetylated

Western blot analysis of extracts from HeLa cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-K18) Antibody. The lane on the left is treated with the synthesized peptide.