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Cyclin B1 polyclonal antibody

货号: BS6874

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6874

Product Name :Cyclin B1 polyclonal antibody

Swiss-Prot :P14635

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background :Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147. At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression. Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CCNB1 polyclonal antibody detects endogenous levels of CCNB1 protein.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :CCNB1; CCNB; cyclin B1; G2/mitotic-specific cyclin-B1

Immunogen :A synthetic peptide of human CCNB1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal (control) and Cyclin B1 knockout (KO) HeLa cells, using Cyclin B1 antibody at 1:1000 dilution.

Western blot analysis of extracts of Mouse liver, using Cyclin B1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat ovary using Cyclin B1 Rabbit pAb at dilution of 1:150 (40x lens).

1-Oxoeudesm-11(13)-eno-12,8a-lactone induces G2/M arrest and apoptosis of human glioblastoma cells in vitro

JournalActa Pharmacologica Sinica

ApplicationWB

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SpeciesHuman

PMID
Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF‐β signalling

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SpeciesHuman

PMID24912879
Dihydroptychantol A, a macrocyclic bisbibenzyl derivative, induces autophagy and following apoptosis associated with p53 pathway in human osteosarcoma U2OS cells

JournalToxicology And Applied Pharmacology

ApplicationWB

IF4.219

SpeciesHuman

PMID
Epigenetic regulation of DACH1, a novel Wnt signaling component in colorectal cancer

JournalEpigenetics

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IF4.173

SpeciesHuman

PMID24149323
MCM-2 is a therapeutic target of Trichostatin A in colon cancer cells

JournalToxicology Letters

ApplicationWB

IF3.569

SpeciesHuamn

PMID23770000