Product Name :ZO1 Recombinant Rabbit mAb
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC, IP
Application_all :WB,1:500 - 1:2000|IHC,1:100 - 1:300|IF/ICC,1:100 - 1:300|IP,1:50 - 1:100
Background :Tight junctions, or zona occludens (ZO), form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins, such as occludin and claudin, to the actin cytoskeleton. ZO-1 and ZO-2 are required for tight junction formation and function . In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators. The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Purification&Purity :The antibody was affinity-purified from cell culture supernatant by immunogen affinity chromatography. and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :ZO1 Recombinant Rabbit mAb detects endogenous levels of ZO1 protein.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :ZO1; Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1
Immunogen :Recombinant fusion protein of human ZO1.
