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p16 INK4a polyclonal antibody

货号: BS66186

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS66186

Product Name :p16 INK4a polyclonal antibody

Swiss-Prot :P42771

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:100

Background :The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. Multiple proteins are encoded by the tumor suppressor gene CDKN2A (MTS1/ p16INK4a) via translation of alternate reading frames, resulting in the production of the p19 ARF protein in mice and the p14 ARF protein in humans. p14 ARF induces an increase in MDM2 and p21 levels and leads to cell cycle arrest in both G1 and G2/M. p14 ARF is negatively regulated by p53 and is known to bind directly to MDM2. CDKN2A also encodes the mitotic protein p16, which binds to and inhibits the Cdk4/cyclin D complex.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p16 INK4a polyclonal antibody detects endogenous levels of p16-INK4a protein.

BiowMW :~ 16 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A; CDKN2A; CDKN2; MTS1

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p16 INK4a.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of p16 INK4a expression in HEK293T (A), HeLa (B), mouse brain (C), rat brain (D) whole cell lysates.

Immunohistochemical analysis of p16 INK4a staining in human colorectal cancer formalin fixed paraffin embedded tissue section.