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HIST1H3A polyclonal antibody

货号: BS65012

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS65012

Product Name :HIST1H3A polyclonal antibody

Swiss-Prot :P68431; Q71DI3; P84243; Q6NXT2

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Diffuse intrinsic pontine glioma (DIPG) is an aggressive brainstem astrocyte tumor arising mostly in children, leading to a long-term survival rate of less than 10%. Multiple whole-genome sequencing studies of DIPG patients identified commonly occurring mutations in the H3F3A gene encoding histone H3.3. One of these mutations, a lysine to methionine amino acid substitution (K27M), is found in up to 78% of DIPGs and 22% of non-brainstem pediatric gliomas. This mutation is associated with poor prognosis, with a mean survival time of 0.73 years for patients with the K27M mutation versus 4.6 years for patients without the mutation. Expression of the K27M mutant histone H3 is accompanied by a dramatic reduction in the levels of polycomb repressive complex 2 (PRC2)-mediated trimethylation of histone H3, changes in the distribution of PRC2 on the genome, and altered expression of genes associated with various cancer pathways.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :HIST1H3A polyclonal antibody detects endogenous levels of HIST1H3A protein.

BiowMW :~ 17 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Histone Lysine Methylation Pathway,Examples of Crosstalk Between Post-translational Modifications,DNA Methylation,

Alternative Name :Histone H3.3;H3-3A;H3.3A, H3F3, H3F3A;PP781;H3-3B;H3.3B, H3F3B

Immunogen :Synthetic peptide, corresponding to Human HIST1H3A.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of HIST1H3A polyclonal antibody at 1:1000 dilution Lane1:COS-7 whole cell lysate(40ug) Lane2:3T3-L1 whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug) Lane5:U-87MG whole cell lysate(40ug) Lane6:Hela whole cell lysate(40ug)