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GRP78 polyclonal antibody

货号: BS6479

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6479

Product Name :GRP78 polyclonal antibody

Swiss-Prot :P11021

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :GRP78 polyclonal antibody detects endogenous levels of GRP78 protein.

BiowMW :~ 78 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; HSPA5; GRP78

Immunogen :Recombinant full length Human GRP7.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using BiP/GRP78 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat brain using BiP/GRP78 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using BiP/GRP78 antibody at dilution of 1:100 (40x lens).

Confocal immunofluorescence analysis of Hela cells using BiP/GRP78 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

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