Product Name :Histone H3 (Mono-Methyl K80) polyclonal antibody
Swiss-Prot :P68431/Q71DI3/P84243/Q6NXT2
Reactivity :Human,Mouse,Rat
Application_all :WB: 1:500~1:1000
Background :Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Histone H3 (Mono-Methyl K80) polyclonal antibody detects endogenous levels of histone H3 only when mono-methylated at Lys80.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Histone Lysine Methylation Pathway,Examples of Crosstalk Between Post-translational Modifications,DNA Methylation,
Alternative Name :CKLF-like MARVEL transmembrane domain-containing protein 3; Chemokine-like factor superfamily member 3; CMTM3; CKLFSF3
Immunogen :Synthetic peptide, corresponding to amino acids 3-63 of Human CMTM3.
