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DFFA polyclonal antibody

货号: BS60819

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS60819

Product Name :DFFA polyclonal antibody

Swiss-Prot :O00273

Host :Rabbit

Reactivity :Human,Rat

Applications :WB

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:203

Background :The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family .

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -23°C long term. Avoid freeze-thaw cycles.

Specificity :DFFA polyclonal antibody detects endogenous levels of DFFA protein.

BiowMW :~ 12 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45; H13

Immunogen :A synthetic peptide corresponding to residues in Human DFFA.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of DFFA polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:H9C2 whole cell lysate