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EIF1 polyclonal antibody

货号: BS60495
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS60495
Product Name :EIF1 polyclonal antibody
Swiss-Prot :P41567
Host :Rabbit
Reactivity :Human,Mouse,Rat
Applications :WB
Application_all :WB: 1:500~1:1000
Background :In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. Eukaryotic translation initiation factor 1 (eIF1) is crucial for the scanning process in vitro. During the scanning process, eIF1 is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of eIF1 in regulating the activity of ribosomal subunits 43S, 48S and 40S. eIF1 enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. It is also a promotor, along with eukar-yotic translation initiation factor 1A (eIF1A), for assembly of 48S ribosomal complexes at the initiation codon of a conventional capped mRNA. In addition, eIF1 and eIF1A, together with eukaryotic translation initiation factor 5 (eIF5), function in the formation of stable 40S ribosomal preinitiation complexes.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :EIF1 polyclonal antibody detects endogenous levels of EIF1 protein.
BiowMW :~ 13 kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Eukaryotic translation initiation factor 1; eIF1; A121; Protein translation factor SUI1 homolog Sui1iso1; EIF1; SUI1
Immunogen :A synthetic peptide corresponding to residues in Human EIF1.
Conjugate :Unconjugated
Modification :Unmodification
Western blot (WB) analysis of EIF1 pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Raw264.7 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:THP-1 whole cell lysate(40ug) Lane5:PC3 whole cell lysate (40ug) Lane6:The lung tissue lysate of Rat (40ug) Lane7:The spleen tissue lysate of Mouse(40ug)

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com