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ENDOG polyclonal antibody

货号: BS60425

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS60425

Product Name :ENDOG polyclonal antibody

Swiss-Prot :Q14249

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria (1). This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA(ssDNA). Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity. Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ENDOG polyclonal antibody detects endogenous levels of ENDOG protein.

BiowMW :~ 35 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Endonuclease G, mitochondrial; Endo G

Immunogen :A synthetic peptide corresponding to residues in Human ENDOG.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ENDOG polyclonal antibody at 1:500 dilution Lane1:HepG2 cell lysate Lane2:Raw264.7 cell lysate Lane3:PC12 cell lysate

免疫沉淀（IP）

免疫组织化学（IHC）

免疫荧光（IF）