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DIAPH2 polyclonal antibody

货号: BS60413

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS60413

Product Name :DIAPH2 polyclonal antibody

Swiss-Prot :O60879

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Dia 1 (also designated p140mDia) and Dia 2 (also designated p134mDia 2), mammalian homologs of the Drosophila diaphanous gene, belong to a family of formin homology (FH) proteins which are characterized by having tandemly aligned FH1 and FH2 domains in their carboxy terminal regions. The amino terminus of inactivated Dia 1 binds to the FH1 and FH2 domains and is activated when GTP-bound Rho disrupts the intramolecular interaction of Dia 1 and converts it to an open conformation. Once activated, Dia 1 co-localizes with Profilin and RhoA in dynamic plasma membrane structures and induces the formation of stress fibers in conjunction with the ROCK-mediated signaling pathway. However, Dia 1 and ROCK activities antagonize each other in order to determine the thickness and density of the stress fibers formed.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :DIAPH2 polyclonal antibody detects endogenous levels of DIAPH2 protein.

BiowMW :~ 130 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Protein diaphanous homolog 2; Diaphanous-related formin-2; DRF2; DIA; Diap2; Dia2; Dia 2

Immunogen :A synthetic peptide corresponding to residues in Human DIAPH2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of DIAPH2 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate

免疫沉淀（IP）

免疫组织化学（IHC）

免疫荧光（IF）