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ACER1 polyclonal antibody

货号: BS60096

价格：￥ ——

货期：现货（1-2个工作日）

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No. :BS60096

Product Name :ACER1 polyclonal antibody

Swiss-Prot :Q8TDN7

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :ASAH3 (N-acylsphingosine amidohydrolase (alkaline ceramidase) 3), also designated ACER1, is a 264 amino acid multi-pass membrane protein that localizes to the membrane of the endoplasmic reticulum. Expressed predominately in epidermal tissue, ASAH3 catalyzes the hydrolysis of the sphingolipid ceramide into sphingosine and a free fatty acid, thereby playing an important role in the regulation of bioactive ceramide and sphingosine levels within the cell. ASAH3 functions at an optimal pH of 8.0 and has high specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine, but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ACER1 polyclonal antibody detects endogenous levels of ACER1 protein.

BiowMW :~ 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Alkaline ceramidase 1; AlkCDase 1; Alkaline CDase 1; EC=3.5.1.23; Acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3; ACER1; ASAH3

Immunogen :A synthetic peptide corresponding to residues in Human ACER1

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ACER1 polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate

Immunohistochemistry (IHC) analyzes of ACER1 pAb bin paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.