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Cytochrome c polyclonal antibody

货号: BS5679

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS5679

Product Name :Cytochrome c polyclonal antibody

Swiss-Prot :P99999

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CYCS pAb detects endogenous levels of CYCS protein.

BiowMW :~ 14 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,AMPK Signaling Pathway,

Alternative Name :Cytochrome c; CYCS; CYC

Immunogen :Synthetic peptide, corresponding Human CYCS.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of CYCS pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:The Embryo tissue lysate of Mouse(40ug) Lane5:The Testis tissue lysate of Rat(40ug)

NG as a novel nitric oxide donor induces apoptosis by increasing reactive oxygen species and inhibiting mitochondrial function in MGC803 cells

JournalInternational Immunopharmacology

ApplicationWB

IF4.932

SpeciesHuman

PMID
Effects of a dammarane-type saponin, ginsenoside Rd, in nicotine-induced vascular endothelial injury

JournalPhytomedicine

ApplicationWB

IF4.268

SpeciesHuman

PMID