Product Name :Hrs (phospho-Y216) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :IHC: 1:50~1:200
Background :Hrs is involved in intracellular trafficking and signal transduction and is associated with early endosomes. Hrs contains a phosphatidylinositol 3-phosphate-binding domain that contributes to its endosomal targeting, where Hrs co-localizes with Clathrin via a Clathrin box motif at the carboxy-terminus of Hrs. Hrs is essential for ventral folding morphogenesis and shares structual similarity to the yeast protein Vps27p, which is involved in vacuolar protein sorting. The human Hrs gene, which maps to chromosome 17q25, enodes a 777 amino acid protein. In Schwann cells, Hrs co-localizes at endosomes with the tumor suppressor protein schwannomin, suggesting a role for schwannomin in Hrs-mediated cell signaling.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-Hrs (Y216) polyclonal antibody detects endogenous levels of Hrs protein when phosphorylated at Tyr216
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Protein pp110; HGS; HRS
Immunogen :Synthetic phosphopeptide derived from human Hrs around the phosphorylation site of Tyrosine 216.
Modification :Phosphorylation
