产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

GAP43 (phospho-S41) polyclonal antibody（KU）

货号: BS4791

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4791

Product Name :GAP43 (phospho-S41) polyclonal antibody（KU）

Swiss-Prot :P17677

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :GAP-43 (growth associated protein 43, B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1 is a crucial component for regenerative response in the nervous system. It is present at high levels in neuronal growth cones during development and axonal regeneration. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The neuron-specific Elav/Hu family member, HuD, interacts with and stabilizes GAP-43 mRNA in developing neurons and leads to increased levels of GAP-43 protein.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-GAP-43 (S41) polyclonal antibody detects endogenous levels of GAP-43 protein only when phosphorylated at Ser41.

BiowMW :~ 25, 48 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Neuronal and Glial Cell Marker Atlas,

Alternative Name :Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46; GAP43

Immunogen :Synthetic phosphopeptide derived from human GAP43 around the phosphorylation site of Serine 41.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-GAP-43 (S41) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with PMA(100nM,30mins) Lane2:Raw264.7 cell lysate treated with PMA(100nM,30mins) Lane3:PC12 cell lysate treated with PMA(100nM,30mins)

Immunohistochemistry (IHC) analyzes of p-GAP43 (S41) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

PTEN–GSK3β–MOB1 axis controls neurite outgrowth in vitro and in vivo

JournalCELLULAR AND MOLECULAR LIFE SCIENCES

ApplicationWB

IF7.014

SpeciesMouse,Rat

PMID30069702