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eIF2α (phospho-S51) polyclonal antibody（KU）

货号: BS4787

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4787

Product Name :eIF2α (phospho-S51) polyclonal antibody（KU）

Swiss-Prot :P05198

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :eIF2α, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2α controls the rate of tRNA translation. When eIF2α is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2α is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-eIF2α (S51) polyclonal antibody detects endogenous levels of eIF2αprotein only when phosphorylated at Ser51.

BiowMW :~ 38 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral Regulation of elF2,

Alternative Name :Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2A; EIF2S1

Immunogen :Synthetic phosphopeptide derived from human eIF2α around the phosphorylation site of Serine 51.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-eIF2α (S51) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:The Uterus tissue lysate of Rat(40ug) Lane4:The Lung tissue lysate of Mouse(40ug) Lane5:A2780 whole cell lysate(40ug) Lane6:H1792 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-eIF2α (S51) pAb in paraffin-embedded human breast carcinoma tissue.

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