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eIF4E (phospho-S209) polyclonal antibody（KU）

货号: BS4748

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4748

Product Name :eIF4E (phospho-S209) polyclonal antibody（KU）

Swiss-Prot :P06730

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-eIF4E (S209) polyclonal antibody detects endogenous levels of eIF4E protein only when phosphorylated at Ser209.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Insulin &Glucose Signaling,

Alternative Name :eIF4E ( phospho S209 ); eIF-4E; eIF4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit; EIF4E; 1977; EIF4E; 133440; P06730; CBP; EIF4EL1; EIF4F; MGC111573;

Immunogen :Synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Serine 209.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-eIF4E (S209) pAb at 1:500 dilution Lane1:The Lung tissue lysate of Mouse(40ug) Lane2:The Brain tissue lysate of Rat(40ug) Lane3:3T3-L1 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:A2780 whole cell lysate(40ug) Lane6:SK-OVCAR3 whole cell lysate(40ug) Lane7:Panc1 whole cell lysate(20ug)

Immunohistochemistry (IHC) analyzes of p-eIF4E (S209) pAb in paraffin-embedded human brain tissue.

15-Deoxy-Δ12,14-prostaglandin J2 induces growth inhibition, cell cycle arrest and apoptosis in human endometrial cancer cell lines

JournalInternational Journal Of Molecular Medicine

ApplicationWB

IF2.928

SpeciesHuman

PMID23403520