产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

IkB-α (phospho-Y42) polyclonal antibody（KU）

货号: BS4736

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4736

Product Name :IkB-α (phospho-Y42) polyclonal antibody（KU）

Swiss-Prot :P25963

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation Serines 32 and 36. IKKα appears to be critical for NFκB activation in response to proinflammatory cytokines. Phosphorylation of IκB by IKKα is stimulated by the NFκB inducing kinase (NIK), which itself is a central regulator for NFκB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ , and each appear to make essential contributions to IκB phosphorylation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-IκB-α (Y42) polyclonal antibody detects endogenous levels of IκB-α protein only when phosphorylated at Tyr42.

BiowMW :~ 36 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,STING Signaling,

Alternative Name :NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI

Immunogen :Synthetic phosphopeptide derived from human IkB-α around the phosphorylation site of Tyrosine 42.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-IkB-α (Y42) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Mouse(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:MCF-7 whole cell lysate(40ug) Lane5:Beas-2B whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-IκB-α (Y42) pAb in paraffin-embedded human breast carcinoma tissue.

The RCAN1 inhibits NF- κ B and suppresses lymphoma growth in mice

JournalCell Death & Disease

ApplicationIP

IF6.304

SpeciesHuman

PMID26492364
Paeonol promotes microRNA-126 expression to inhibit monocyte adhesion to ox-LDL-injured vascular endothelial cells and block the activation of the PI3K/Akt/NF-κB pathway

JournalInternational Journal Of Molecular Medicine

ApplicationWB

IF2.928

SpeciesRat

PMID27748840
Anti-oxidant and Anti-inflammatory Effects of Ethanol Extract from Polygala sibirica L. var megalopha Fr. on Lipopolysaccharide-Stimulated RAW264.7 Cells

JournalChinese Journal of Integrative Medicine

ApplicationWB

IF2.9

SpeciesMouse

PMID37434032
Gualou Xiebai Decoction ameliorates cardiorenal syndrome type II by regulation of PI3K/AKT/NF-κB signalling pathway

JournalPHYTOMEDICINE

ApplicationWB

IF7.9

SpeciesRat

PMID37976694