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IL-10Rα (phospho-Y496) polyclonal antibody（KU）

货号: BS4592

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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No. :BS4592

Product Name :IL-10Rα (phospho-Y496) polyclonal antibody（KU）

Swiss-Prot :Q13651

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to phosphorylation of JAK1 and Tyk 2 tyrosine kinases. The activated kinases phosphorylate the two tyrosine residues (Tyr 446 and Tyr 496) in the cytoplasmic domain of IL-10Rα. The phosphorylation of these two residues are required for proper function of IL-10R and activation of IL-10E1 signaling. IL-10Rβ is ubiquitously expressed and, in addition to forming the IL-10 heterodimeric receptor, it forms a heterodimeric receptor with an IL-22R subunit and an IL-28R subunit. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-IL-10Rα (Y496) pAb detects endogenous levels of IL-10Rα protein only when phosphorylated at Tyr496.

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IL-10R-A; IL-10R1; CDw210a antigen; IL10RA; IL10R

Immunogen :Synthetic phosphopeptide derived from human IL-10Rα around the phosphorylation site of Tyrosine 496

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-IL-10Rα (Y496) pAb at 1:500 dilution Lane1:Myla2059 whole cell lysate(40µg) Lane2:K562 whole cell lysate(40µg) Lane3:RAW264.7 whole cell lysate(40µg) Lane4:C6 whole cell lysate(40µg)

Activation of Paraventricular Melatonin Receptor 2 Mediates Melatonin-Conferred Cardioprotection Against Myocardial Ischemia/Reperfusion Injury

JournalJournal Of Cardiovascular Pharmacology

ApplicationWB

IF2.598

SpeciesMouse

PMID32433359