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EGFR (phospho-Y992) polyclonal antibody（KU）

货号: BS4310

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4310

Product Name :EGFR (phospho-Y992) polyclonal antibody（KU）

Swiss-Prot :P00533

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Epidermal growth factor mediates its effects on cell growth through its interaction with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGFα to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy-terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptorgulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-EGFR (Y992) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at Tyr992 (removal of the signal peptide).

BiowMW :~ 175 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling,

Alternative Name :Epidermal growth factor receptor; EC=2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; EGFR

Immunogen :Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Tyrosine 992.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-EGFR (Y992)) pAb at 1:500 dilution Lane1:HCC827 whole cell lysate(20ug) Lane2:A549 whole cell lysate(40ug)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p-EGFR (Y992) pAb. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using p-EGFR (Y992) pAb. The picture on the right is treated with the synthesized peptide.