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AKT1 (phospho-S246) polyclonal antibody

货号: BS4286
价格 780
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS4286
Product Name :AKT1 (phospho-S246) polyclonal antibody
Swiss-Prot :P31749
Host :Rabbit
Reactivity :Human,Mouse,Rat
Applications :WB IHC
Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200
Background :AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-AKT1 (S246) polyclonal antibody detects endogenous levels of AKT1 protein only when phosphorylated at Ser246.This antibody does not cross-react with AKT2 or AKT3.
BiowMW :~ 60 kDa
Note :For research use only, not for use in diagnostic procedure.
Pathway :Phosphoinosotode Signaling,PI3K AKT signaling,Protein Kinase C Signaling,Warburg Effect,T cell receptor signaling,Inhibition of Apoptosis,Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,Adherens Junction signaling,Mitochondrial Control of Apoptosis,MTOR signaling,ErbB HER Signaling,
Alternative Name :RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα;
Immunogen :Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Serine 81.
Conjugate :Unconjugated
Modification :Phosphorylation
Western blot (WB) analysis of AKT1 (phospho-S246) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with EGF(0.1ng/ml,5min) whole cell lysate Lane3:L02 treated with EGF(0.1ng/ml,10min) whole cell lysate Lane4:L02 treated with EGF(0.1ng/ml,15min) whole cell lysate Lane5:The Heart tissue lysate of Mouse(40ug) Lane6:The fetal Heart tissue lysate of Rat(40ug)
Immunohistochemistry (IHC) analyzes of p-AKT1 (S246) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nuclear and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Immunohistochemistry (IHC) analyzes of AKT1 (phospho-S246) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • LFG-500 Inhibits the Invasion of Cancer Cells via Down-Regulation of PI3K/AKT/NF-κB Signaling Pathway
    JournalPLoS One
    ApplicationWB
    IF2.74
    SpeciesHuman
  • Diversity in human placental microvascular endothelial cells and macrovascular endothelial cells
    JournalCytokine
    ApplicationWB
    IF2.952
    SpeciesHuman

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com