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FoxO1/3/4 (phospho-T24/32) polyclonal antibody（KU）

货号: BS4266

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4266

Product Name :FoxO1/3/4 (phospho-T24/32) polyclonal antibody（KU）

Swiss-Prot :Q12778/O43524/P98177

Host :Rabbit

Reactivity :Human

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors.FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined.FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-FoxO1/3/4 (T24/32) polyclonal antibody detects endogenous levels of FoxO1 protein only when phosphorylated at Thr24. This antibody also detects endogenous levels of FoxO3 and FoxO4 protein only when phosphorylated at Thr32.

BiowMW :~ 70 to 82 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,

Alternative Name :Forkhead box protein O4; Fork head domain transcription factor AFX1; AFX; AFX1; MLLT7; FOXO4; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO1; FKHR; FOXO1A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1; FOXO3A

Immunogen :Synthetic phosphopeptide derived from human FoxO1 around the phosphorylation site of Threonine 24.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-FoxO1/3/4 (T24/32) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:A2780 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-FoxO1/3/4 (T24/32) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Casticin prevents DSS induced ulcerative colitis in mice through inhibitions of NF‐κB pathway and ROS signaling

JournalPhytotherapy Research

ApplicationWB,IF

IF3.766

SpeciesHuman

PMID29876982