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GRF-1 (phospho-Y1105) polyclonal antibody （KU）

货号: BS4232

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4232

Product Name :GRF-1 (phospho-Y1105) polyclonal antibody （KU）

Swiss-Prot :Q9NRY4

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :GRF-1 (glucocorticoid receptor DNA-binding factor 1), also known as p190RhoGAP or simply p190, is a transcriptional regulator which binds to the promoter region of the glucocorticoid receptor gene and represses its expression. By repressing GR expression, GRF-1 acts to down-regulate Rho signaling, thereby mediating both actin cytoskeletal rearrangements and cell cycle events. Through its GAP domain, GRF-1 is thought to affect cytokinesis by regulating Rho activity; a regulation that is controlled by the ubiquination of the GTP binding region and subsequent degradation of GRF-1. Additionally, GRF-1 plays an important role in oligodendrocyte differentiation, a process that is absent in malignant glioma tumors, implicating GRF-1 as a possible tumor suppressor. GRF-1 expression is regulated by glucocorticoids and the expressed protein exists as two isoforms produced by alternative splicing events.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-GRF-1 (Y1105) polyclonal antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Tyr1105.

BiowMW :~ 172 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A; ARHGAP35; GRF1; GRLF1; KIAA1722

Immunogen :Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1105.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-GRF-1 (Y1105) pAb at 1:500 dilution Lane1:The Heart tissue lysate of Rat(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug) Lane4:The Blood tissue lysate of Mouse(40ug) Lane5:H1792 whole cell lysate(40ug) Lane6:HEK293T whole cell lysate(40ug) Lane7:SP2/0 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-GRF-1 (Y1105) pAb in paraffin-embedded human brain tissue.