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AMPKβ1 (phospho-S181) polyclonal antibody

货号: BS4213

价格：￥ 780

货期：现货（1-2个工作日）

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No. :BS4213

Product Name :AMPKβ1 (phospho-S181) polyclonal antibody

Swiss-Prot :Q9Y478

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :AMPKβ1 is a member of the SNF1 kinase family. It is a heterotrimeric protein comprising of an alpha (catalytic), beta (non-catalytic) and gamma (non-catalytic) subunits, 63, 38 and 38kDa respectively.AMPKβ1 regulates fatty acid and sterol synthesis by phosphorylation of acteyl-coA. It also regulates cholesterol synthesis via phosphorylation and in-activation of hydroxymethylglutaryl-CoA reductase.Two isoforms of AMPKβ1 have been found.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-AMPKβ1 (S181) polyclonal antibody detects endogenous levels of AMPKβ1 protein only when phosphorylated at Ser181

BiowMW :~ 38 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Hypoxia Signaling,AMPK Signaling Pathway,MTOR signaling,

Alternative Name :5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb; PRKAB1; AMPK; AMPK β1

Immunogen :Synthetic phosphopeptide derived from human AMPKβ1 around the phosphorylation site of Serine 181.

Conjugate :Unconjugated

Modification :Phosphorylation

Immunohistochemistry (IHC) analyzes of p-AMPKβ1 (S181) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of p-AMPKβ1 (S181) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of p-AMPKβ1 (S181) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.