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IKKα (phospho-T23) polyclonal antibody（KU）

货号: BS4100

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS4100

Product Name :IKKα (phospho-T23) polyclonal antibody（KU）

Swiss-Prot :O15111

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IKKα (phospho-T23) polyclonal antibody detects endogenous levels of IKKα protein only when phosphorylated at Thr23.

BiowMW :~ 85 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,PI3K AKT signaling,Immune Cell Signaling,STING Signaling,Inhibition of Apoptosis,

Alternative Name :I kappa-B kinase alpha; IkBKA; IKK-alpha; IKK-A; IkappaB kinase; EC 2.7.11.10; I-kappa-B kinase 1; IKK1; Conserved helix-loop-helix ubiquitous kinase; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; CHUK; IKKA; TCF16

Immunogen :Synthetic phosphopeptide derived from human IKKα around the phosphorylation site of Threonine 23.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of IKKα (phospho-T23) polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:The Embryo tissue lysate of Rat(10ug) Lane5:The Brain tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of IKKα (phospho-T23) polyclonal antibody in paraffin-embedded human colorectal carcinoma tissue at 1:50.

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