Product Name :HDAC8 (phospho-S39) polyclonal antibody
Reactivity :Human,Mouse,Rat;
Application_all :IHC: 1:50~1:200
Background :In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-HDAC8 (S39) polyclonal antibody detects endogenous levels of HDAC8 protein only when phosphorylated at Ser39.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Cell Cycle Control G1 S Checkpoint,
Alternative Name :Histone deacetylase 8; HD8; HDAC8; HDACL1; CDA07
Immunogen :Synthetic phosphopeptide derived from human HDAC8 around the phosphorylation site of Serine 39.
Modification :Phosphorylation
