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GSK3α/β (phospho-Y279/216) polyclonal antibody（KU）

货号: BS4083

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS4083

Product Name :GSK3α/β (phospho-Y279/216) polyclonal antibody（KU）

Swiss-Prot :P49840/P49841

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:2000 IHC: 1:50~1:200

Background :Glycogen synthase kinase-3 α and β (GSK-3α, β) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase)-protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-GSK3α/β (Y279/216) polyclonal antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Tyr279/216

BiowMW :~ 46,51 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :PI3K AKT signaling,Hedgehog Signaling,Hypoxia Signaling,Translational Contral elF4 and p70 S6 Kinase,Translational Contral Regulation of elF2,Regulation of Microtubule Dynamics,Inhibition of Apoptosis,Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,

Alternative Name :Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A

Immunogen :Synthetic phosphopeptide derived from human GSK3α around the phosphorylation site of Tyrosine 279.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of GSK3α/β (phospho-Y279/216) polyclonal antibody at 1:2000 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with PBS(1×PBS,PH7.4) for 30min whole cell lysate Lane3:L02 treated with PBS(1×PBS,PH7.4) for 60min whole cell lysate Lane4:L02 treated with PBS(1×PBS,PH7.4) for 120min whole cell lysate Lane5:L02 treated with PBS(1×PBS,PH7.4) for 180min whole cell lysate Lane6:L02 treated with PBS(1×PBS,PH7.4) for 240min whole cell lysate Lane7:C6 whole cell lysate

Immunohistochemistry (IHC) analyzes of p-GSK3α/β (Y279/216) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

l-Theanine attenuates cadmium-induced neurotoxicity through the inhibition of oxidative damage and tau hyperphosphorylation

JournalNeurotoxicology

ApplicationWB

IF3.105

SpeciesMouse

PMID27649883