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GR (phospho-S226) polyclonal antibody

货号: BS4081

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4081

Product Name :GR (phospho-S226) polyclonal antibody

Swiss-Prot :P04150

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR α. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-GR (S226) polyclonal antibody detects endogenous levels of GR protein only when phosphorylated at Ser226

BiowMW :~ 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Nuclear Receptor Signaling,Nuclear Receptor Signaling,

Alternative Name :Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1; NR3C1; GRL

Immunogen :Synthetic phosphopeptide derived from human GR around the phosphorylation site of Serine 226.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-NR3C1(S226/234/246) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate(40ug) Lane2:SGC7901 treated with UV for 5 minutes then repair for 1 hour whole cell lysate(40ug) Lane3:SGC7901 treated with UV for 5 minutes then repair for 3 hours whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-GR (S226) pAb in paraffin-embedded human lung carcinoma tissue.