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Glycogen synthase 1(Phospho-S645) polyclonal antibody（KU）

货号: BS4080

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4080

Product Name :Glycogen synthase 1(Phospho-S645) polyclonal antibody（KU）

Swiss-Prot :P13807

Host :Rabbit

Reactivity :Human,Rat

Applications :WB

Application_all :WB: 1:1000~1:2000

Background :Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver. Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals -- muscle isoform (GYS1) and liver isoform (GYS2) respectively. Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649 and Ser653) inhibiting its activity. Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth.

Product :1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Glycogen synthase 1(Phospho-S645) polyclonal antibody detects endogenous levels of Glycogen synthase 1 protein only when phosphorylated at Ser645.

BiowMW :~ 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Insulin &Glucose Signaling,

Alternative Name :Glycogen [starch] synthase, muscle; GYS1; GYS

Immunogen :Synthetic phosphopeptide derived from human Glycogen synthase 1 around the phosphorylation site of Serine 645.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Glycogen synthase 1(Phospho-S645) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:Hela treated with insulin(1μM/L,5 minutes) whole cell lysate(40ug) Lane3:Hela treated with insulin(1μM/L,10 minutes) whole cell lysate(40ug) Lane4:The Stomach tissue lysate of Rat(40ug)