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EEF2 (phospho-T56) polyclonal antibody（KU）

货号: BS4059

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS4059

Product Name :EEF2 (phospho-T56) polyclonal antibody（KU）

Swiss-Prot :P13639

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-EEF2 (T56) polyclonal antibody detects endogenous levels of EF-2 protein when phosphorylated at Thr56.

BiowMW :~ 95 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Elongation factor 2; EF-2; EEF2; EF2

Immunogen :Synthetic peptide, corresponding to amino acids 20-70 of Human eEF2.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-EEF2 (T56) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(10ug) Lane3:The Liver tissue lysate of Rat(40ug) Lane4:The Liver tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of p-EEF2 (T56) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

eEF2K promotes progression and radioresistance of esophageal squamous cell carcinoma

JournalRadiotherapy And Oncology

ApplicationWB

IF5.252

SpeciesHuman

PMID28431753