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DARPP-32 (phospho-T75) polyclonal antibody（KU）

货号: BS4058

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4058

Product Name :DARPP-32 (phospho-T75) polyclonal antibody（KU）

Swiss-Prot :Q9UD71

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson’s disease and drug abuse. DARPP-32 (for dopamine and adenosine 3’,5’-monophosphate-regulated phosphoprotein of 32 kDa) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-DARPP-32 (T75) polyclonal antibody detects endogenous levels of DARPP-32 protein only when phosphorylated at Thr75

BiowMW :~ 32 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Neuronal and Glial Cell Marker Atlas,Dopamine Signaling in Parkinson's Disease Plathway,

Alternative Name :Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; PPP1R1B; DARPP32

Immunogen :Synthetic phosphopeptide derived from human DARPP-32 around the phosphorylation site of Threonine 75.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-DARPP-32 (T75) pAb at 1:1000 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:SGC7901 whole cell lysate(40ug) Lane5:PC3 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-DARPP-32 (T75) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.