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Cyclin E1 (phospho-T395) polyclonal antibody（KU）

货号: BS4057

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4057

Product Name :Cyclin E1 (phospho-T395) polyclonal antibody（KU）

Swiss-Prot :P24864

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Cyclin E1 (T395) polyclonal antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at Thr395

BiowMW :~ 48 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :G1/S-specific cyclin-E1; CCNE1; CCNE

Immunogen :Synthetic phosphopeptide derived from human Cyclin E1 around the phosphorylation site of Threonine 395.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Cyclin E1 (T395) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) Lane4:BV2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-Cyclin E1 (T395) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.