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Chk2 (phospho-T68) polyclonal antibody

货号: BS4043

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4043

Product Name :Chk2 (phospho-T68) polyclonal antibody

Swiss-Prot :O96017

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1..

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Chk2 (T68) polyclonal antibody detects endogenous levels of Chk2 protein when phosphorylated at Thr68.

BiowMW :~ 62 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle Control G1 S Checkpoint,Examples of Crosstalk Between Post-translational Modifications,Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2; CHEK2; CDS1; CHK2; RAD53

Immunogen :Synthetic phosphopeptide derived from human Chk2 around the phosphorylation site of Threonine 68.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Chk2 (T68) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with UV Lane2:Mouse kidney tissue lysate Lane3:H9C2 cell lysate treated with UV

Immunohistochemistry (IHC) analyzes of p-Chk2 (T68) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Rad9a is involved in chromatin decondensation and post-zygotic embryo development in mice

JournalCell Death And Differentiation

ApplicationIF

IF10.717

SpeciesMouse

PMID30154445
Ablation of beta subunit of protein kinase CK2 in mouse oocytes causes follicle atresia and premature ovarian failure

JournalCell Death & Disease

ApplicationWB

IF6.304

SpeciesMouse

PMID29725001
CHEK2 signaling is the key regulator of oocyte survival after chemotherapy

JournalScience Advances

ApplicationIF

IF13.6

SpeciesMouse

PMID37862420