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Chk1 (phospho-S317) polyclonal antibody

货号: BS4040

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4040

Product Name :Chk1 (phospho-S317) polyclonal antibody

Swiss-Prot :O14757

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Chk1 (S317) polyclonal antibody detects endogenous levels of Chk1 protein when phosphorylated at Ser317.

BiowMW :~ 56 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle Control G1 S Checkpoint,Cell Cycle G2 M DNA Damage Signaling Pathway,Epigenetic Writers and Erasers of Histone H3,

Alternative Name :Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1; CHEK1; CHK1

Immunogen :Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 317.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Chk1 (phospho-S317) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate Lane3:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate

Immunohistochemistry (IHC) analyzes of p-Chk1 (S317) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing nuclear staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

VPA mediates bidirectional regulation of cell cycle progression through the PPP2R2A-Chk1 signaling axis in response to HU

JournalCell Death & Disease

ApplicationIHC

IF9.685

SpeciesRat

PMID36781846