中国 EN 400-660-9091

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

AKT (phospho-S473) polyclonal antibody

货号: BS4006
价格 780
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS4006
Product Name :AKT (phospho-S473) polyclonal antibody
Swiss-Prot :P31749/P31751/Q9Y243
Host :Rabbit
Reactivity :Human,Mouse,Rat
Applications :WB IHC
Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200
Background :AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-Akt (S473) polyclonal antibody detects endogenous levels of p-Akt protein only when phosphorylated at Ser473.
BiowMW :~ 60 kDa
Note :For research use only, not for use in diagnostic procedure.
Pathway :Phosphoinosotode Signaling,PI3K AKT signaling,Protein Kinase C Signaling,Immune Cell Signaling,Warburg Effect,Translational Contral elF4 and p70 S6 Kinase,Regulation of Microtubule Dynamics,Regulation of Microtubule Dynamics,T cell receptor signaling,Inhibition of Apoptosis,Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,Adherens Junction signaling,Mitochondrial Control of Apoptosis,MTOR signaling,ErbB HER Signaling,
Alternative Name :AKT1; AKT1; AKT2; AKT3; MGC9965; Oncogene AKT1; PKB alpha; PKB; PKB beta; PKBalpha; PRKBA; PRKBB; PRKBG; Protein kinase B; Protein kinase B beta; Protein kinase B gamma; Proto-oncogene c-Akt; RAC alpha; RAC; RAC gamma; RAC PK alpha; RAC PK beta; RAC
Conjugate :Unconjugated
Modification :Phosphorylation
Western blot (WB) analysis of p-AKT (S473) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Mouse(40ug) Lane2:The Lung tissue lysate of Rat(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug) Lane5:L02 whole cell lysate(40ug)
Immunohistochemistry (IHC) analyzes of p-Akt (S473) pAb in paraffin-embedded human breast carcinoma tissue.
  • Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway
    JournalOncology Reports
    ApplicationWB
    IF3.417
    SpeciesHuman
  • MicroRNA-34a Suppresses Cell Proliferation by Targeting LMTK3 in Human Breast Cancer MCF-7 Cell Line
    JournalDna And Cell Biology
    ApplicationWB
    IF3.311
    SpeciesHuman
    PMID
  • 5,7-Dihydroxyflavone Enhances the Apoptosis-Inducing Potential of TRAIL in Human Tumor Cells via Regulation of Apoptosis-Related Proteins
    JournalEvidence-based Complementary and Alternative Medicine
    ApplicationWB
    IF2.629
    SpeciesHuman
    PMID
  • Monoclonal antibody to six transmembrane epithelial antigen of prostate-4 influences insulin sensitivity by attenuating phosphorylation of P13K (P85) and Akt: Possible mitochondrial mechanism
    JournalJournal Of Bioenergetics And Biomembranes
    ApplicationWB
    IF2.524
    SpeciesHuman
  • Activation of GPER suppresses epithelial mesenchymal transition of triple negative breast cancer cells via NF-κB signals
    JournalMolecular Oncology
    ApplicationWB
    IF6.574
    SpeciesHuman

关注我们

400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com