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eIF3ζ (R135) polyclonal antibody（KU）

货号: BS3860

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS3860

Product Name :eIF3ζ (R135) polyclonal antibody（KU）

Swiss-Prot :O15371

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFε, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :eIF3ζ (R135) polyclonal antibody detects endogenous levels of eIF3ζ protein.

BiowMW :~ 64 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Eukaryotic translation initiation factor 3 subunit D; eIF3d; Eukaryotic translation initiation factor 3 subunit 7; eIF-3-zeta; eIF3 p66; EIF3S7; EIF3D

Immunogen :Synthetic peptide, corresponding to amino acids 111-160 of Human eIF3ζ.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of eIF3ζ (R135) pAb at 1:1000 dilution Lane1:The Brain tissue lysate of Mouse(40ug) Lane2:The Brain tissue lysate of Rat(20ug) Lane3:HEK293T whole cell lysate(10ug) Lane4:MCF-7 whole cell lysate(20ug)

Immunohistochemistry (IHC) analyzes of eIF3ζ (R135) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.