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GCS-α-1 (D423) polyclonal antibody（KU）

货号: BS3670

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS3670

Product Name :GCS-α-1 (D423) polyclonal antibody（KU）

Swiss-Prot :Q02108

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase, a soluble form (GCS or sGC), which act as receptors for nitric oxide and a membrane-bound receptor form (GC), which are peptide hormone receptors. The GC-C protein is composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic region consisting of a kinase-like domain and a catalytic domain. It is expressed as two differentially glycosylated forms, a 130 kDa precursor form present in the endoplasmic reticulum and a 145 kDa form present on the plasma membrane. Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP. GC-C acts as the receptor for heatstable enterotoxins, small peptides secreted by some pathogenic strains of E. coli that cause severe secretory diarrhea. GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :GCS-α-1 (D423) polyclonal antibody detects endogenous levels of GCS-α-1 protein.

BiowMW :~ 77 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Guanylate cyclase soluble subunit alpha-3; GCS-alpha-3; EC=4.6.1.2; GCS-alpha-1; Soluble guanylate cyclase large subunit; GUC1A3; GUCSA3; GUCY1A1; GUCY1A3; GCSα1; GCS-α1

Immunogen :Synthetic peptide, corresponding to amino acids 400-450 of Human GCS-α-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of GCS-α-1 (D423) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(20ug) Lane2:A549 whole cell lysate(20ug) Lane3:HEK293T whole cell lysate(20ug) Lane4:PMVEC whole cell lysate(40ug) Lane5:The Brain tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of GCS-α-1 (D423) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.