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FAK (I855) polyclonal antibody（KU）

货号: BS3583

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS3583

Product Name :FAK (I855) polyclonal antibody（KU）

Swiss-Prot :Q05397

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100

Background :Focal adhesion kinase was initially identified as a major 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :FAK (I855) polyclonal antibody detects endogenous levels of FAK protein.

BiowMW :~ 125 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Tumor Angiogenesis,Contribution of ECM and Cytoskeletal Factors to EMT,G Protein-coupled Receptors Signaling to MAPK Erk,

Alternative Name :Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK; PTK2; FAK; FAK1

Immunogen :Synthetic peptide, corresponding to amino acids 830-880 of Human FAK.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of FAK (I855) pAb at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of FAK (I855) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunoprecipitation of HEK293T cell lysate using FAK (I855) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 ) .Lane 1 is 30% input. The western blot was probed using FAK (I855). “↑”（supernatant）; “↓(deposition）

PTK2-mediated degradation of ATG3 impedes cancer cells susceptible to DNA damage treatment

JournalAutophagy

ApplicationWB

IF11.059

SpeciesHuman

PMID28103122
ADAM23 in Cardiomyocyte Inhibits Cardiac Hypertrophy by Targeting FAK‐AKT Signaling

JournalJournal of the American Heart Association

ApplicationWB

IF4.66

SpeciesMouse

PMID30371220