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Cytokeratin 19 (L349) polyclonal antibody（KU）

货号: BS3540

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS3540

Product Name :Cytokeratin 19 (L349) polyclonal antibody（KU）

Swiss-Prot :P08727

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cytokeratin 19 (L349) polyclonal antibody detects endogenous levels of Cytokeratin 19 protein.

BiowMW :~ 44 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of Soluble Factors to EMT,

Alternative Name :Keratin, type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19; KRT19

Immunogen :Synthetic peptide, corresponding to amino acids 321-370 of Human Cytokeratin 19.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Cytokeratin 19 (L349) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug) Lane4:NIH-3T3 whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Cytokeratin 19 (L349) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Fatty acid synthase-positive hepatocytes and subsequent steatosis in rat livers by irinotecan

JournalOncology Reports

ApplicationIHC

IF3.041

SpeciesRat

PMID25708528