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AQP0 (C144) polyclonal antibody（KU）

货号: BS3508

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS3508

Product Name :AQP0 (C144) polyclonal antibody（KU）

Swiss-Prot :P30301

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :AQP0 (C144) polyclonal antibody detects endogenous levels of AQP0 protein.

BiowMW :~ 28 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26; MIP; AQP0; Aquaporin 0; Aquaporin0;

Immunogen :Synthetic peptide, corresponding to amino acids 110-160 of Human AQP0.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of AQP0 (C144) pAb at 1:500 dilution Lane1:THP-1 whole cell lysate(40ug) Lane2:L02 whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of AQP0 (C144) pAb in paraffin-embedded human brain tissue.

Molecular mechanism of Aquaporin 0-induced fiber cell to fiber cell adhesion in the eye lens

JournalBiochemical And Biophysical Research Communications

ApplicationIF

IF3.575

SpeciesMouse

PMID30348525