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IFN-γ (Q69) polyclonal antibody

货号: BS3486

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3486

Product Name :IFN-γ (Q69) polyclonal antibody

Swiss-Prot :P01579

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :Interferon (IFN)-γ is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. IFN-γ production modulates T cell growth and differentiation and inhibits the growth of B cells. Synthesis of IFN-γ is inducible by IL-2, FGF and EGF. The active form of IFN-γ is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-γ is stabilized by non-covalent interactions through the interface of the helices. IFN-γ tranlsated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IFN-γ (Q69) polyclonal antibody detects endogenous levels of IFN-γ protein.

BiowMW :~ 19 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IFN-gamma; Immune interferon; IFN-gamma . Interferon gamma; IFNG; IFNγ

Immunogen :Synthetic peptide, corresponding to amino acids 31-80 of Human IFN-γ.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of IFN-γ (Q69) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate

Immunohistochemistry (IHC) analyzes of IFN-γ (Q69) pAb in paraffin-embedded human lymph node tissue.

Immunoprecipitation of the Spleen tissue lysate of Rat using IFN-γ (Q69) pAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using IFN-γ (Q69) pAb #BS3486.“↑”(supernatant); “↓”(deposition)

EBV-Encoded miR-BART20-5p and miR-BART8 Inhibit the IFN-γ–STAT1 Pathway Associated with Disease Progression in Nasal NK-Cell Lymphoma

JournalAmerican Journal Of Pathology

ApplicationWB

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SpeciesHuman

PMID24655378
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Arctigenin exerts anti-colitis efficacy through inhibiting the differentiation of Th1 and Th17 cells via an mTORC1-dependent pathway

JournalBiochemical Pharmacology

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Respiratory Syncytial Virus Infection Upregulates NLRC5 and Major Histocompatibility Complex Class I Expression through RIG-I Induction in Airway Epithelial Cells

JournalJournal Of Virology

ApplicationWB

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SpeciesHuman

PMID25972545
Clostridium butyricum B1 alleviates high-fat diet-induced steatohepatitis in mice via enterohepatic immunoregulation

JournalJournal Of Gastroenterology And Hepatology

ApplicationIHC

IF4.029

SpeciesMouse

PMID28109017