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Aldolase B (W148) polyclonal antibody（KU）

货号: BS3127

价格：￥ 660

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3127

Product Name :Aldolase B (W148) polyclonal antibody（KU）

Swiss-Prot :P05062

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:2000 IHC: 1:50~1:200

Background :Aldolase A is one of the most highly conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagons and has been implicated in hereditary fructose intolerance disease. Aldolase C is 36 kDa polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Aldolase B (W148) polyclonal antibody detects endogenous levels of Aldolase B protein.

BiowMW :~ 40 to 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ALDOB; ALDOB; ALDB; Liver-type aldolase; Fructose-bisphosphate aldolase B

Immunogen :Synthetic peptide, corresponding to amino acids 121-170 of Human Aldolase B.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Aldolase B (W148) pAb at 1:2000 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:The Kidney tissue lysate of Rat(10ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(20ug) Lane5:SGC7901 whole cell lysate(20ug)

Immunohistochemistry (IHC) analyzes of Aldolase B (W148) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.