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ERdj3 (K66) polyclonal antibody（KU）

货号: BS3114

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3114

Product Name :ERdj3 (K66) polyclonal antibody（KU）

Swiss-Prot :Q9UBS4

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100

Background :Members of the heat shock protein 40 (HSP 40) family of proteins all contain a highly conserved J domain that associates with HSP 70 and regulates the function of HSP 70 by activating its adenosine triphosphatase activity. ERdj3, an HSP 40 chaperone, is expressed in the ER lumen, where it interacts with BiP, a molecule involved in retrotranslocating proteins out of the ER. ERdj3 also associates with several other protein substrates, including unfolded light chains, a nonsecreted Ig light chain mutant and a VSV-G ts045 mutant. Shiga toxin (Stx) is a bacterial tool that enzymatically inactivates the 28S rRNA, inhibiting protein synthesis of infected cells. Stx also interacts with ERdj3 and Sec 61 to form a complex through which proteins are retrotranslocated to the cytoplasm. ERdj3 may play a role in the ER quality control system.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ERdj3 (K66) polyclonal antibody detects endogenous levels of ERdj3 protein.

BiowMW :~ 40 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DnaJ homolog subfamily B member 11; APOBEC1-binding protein 2; ABBP-2; ABBP2; DnaJ protein homolog 9; ER-associated DNAJ; ER-associated Hsp40 co-chaperone; Endoplasmic reticulum DNA J domain-containing protein 3; ER-resident protein ERdj3; ERdj3; ERj3p; HEDJ; Human DnaJ protein 9; hDj-9; PWP1-interacting protein 4; DNAJB11; EDJ; ERJ3; HDJ9; PSEC0121; UNQ537/PRO1080

Immunogen :Synthetic peptide, corresponding to amino acids 32-88 of Human ERdj3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ERdj3 (K66) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PANC1 whole cell lysate(40ug) Lane3:The Lung tissue lysate of Mouse(40ug) Lane4:The Kidney tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of ERdj3 (K66) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Immunoprecipitation of L02 whole cell lysate using ERdj3 (K66) pAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5 ) .Lane 1 is 20% input. The western blot was probed using ERdj3 (K66) pAb #BS3114.“↑”（supernatant）; “↓”（deposition）