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Arfaptin 1 (K314) polyclonal antibody（KU）

货号: BS3076

价格：￥ 660

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS3076

Product Name :Arfaptin 1 (K314) polyclonal antibody（KU）

Swiss-Prot :P53367

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Arfaptin 1 (K314) polyclonal antibody detects endogenous levels of Arfaptin 1 protein.

BiowMW :~ 40 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1; Arfaptin1

Immunogen :Synthetic peptide, corresponding to amino acids 300-350 of Human Arfaptin 1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Arfaptin 1 (K314) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:SP2/0 cell lysate Lane3:PC12 cell lysate Lane4:Rat muscle tissue lysate

Immunohistochemistry (IHC) analyzes of Arfaptin 1 (K314) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.