Product Name :Id4 (S5) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :IHC:1:50~1:200
Background :Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1 , Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Id4 (S5) polyclonal antibody detects endogenous levels of Id4 protein.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :DNA-binding protein inhibitor ID-4; Class B basic helix-loop-helix protein 27; bHLHb27; Inhibitor of DNA binding 4; ID4; BHLHB27
Immunogen :Synthetic peptide, corresponding to amino acids N-terminus of Human Id4.
Modification :Unmodification
